A9941
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A9941
Sigma
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
tablet, 10 mg substrate per tablet
| Synonym: | AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) |
| CAS Number: | 30931-67-0 |
| Linear Formula: | C18H24N6O6S4 |
| Molecular Weight: | 548.68 |
| Beilstein Registry Number: | 7329461 |
| EC Number: | 250-396-6 |
| MDL number: | MFCD00010404 |
| PubChem Substance ID: | 24891410 |
Description
| Application | 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications. |
| Reconstitution | Dissolve 1 tablet in 100 ml of 0.05 M phosphate-citrate buffer, pH 5.0 (Tablets available as Sigma Product No. P4809). Add 25 μl of fresh 30% hydrogen peroxide |
Properties
| form | tablet |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Gloves |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| WGK Germany | 3 |
| RTECS | DL7002000 |
